Excel · study · 2026
Two mycorrhiza-responsive MADS transcription factors, OsMADS61 and OsMADS26, regulate both direct and mycorrhizal nitrate transport pathways
Listed in NCBI GEO
Most land plants have evolved both a direct root uptake pathway and a symbiotic pathway, via association with arbuscular mycorrhizal (AM) fungi, to facilitate nutrient acquisition, particularly of phosphorus (P) and nitrogen (N), from soil.
Description
Recently, we revealed a highly efficient symbiotic pathway for nitrate uptake, mediated by an AM-specific NPF/NRT1 transporter, OsNPF4.5, in rice. However, the regulatory mechanism controlling the AM-specialized expression of OsNPF4.5 remains unclear.
Here, we demonstrate that two cis-acting elements, the CArG and GCC-box, are essential for activating the expression of OsNPF4.5 in rice mycorrhizal roots. Deletion of either of the two motifs in its promoter caused almost complete abolition of the promoter activity of OsNPF4.5. An AM-responsive MADS (MCM1, AG, DEFA, and SRF) transcript factor, OsMADS61, could positively regulate OsNPF4.5 and another nitrate transporter gene, OsNRT2.2, involved in direct nitrate uptake.
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Knockout of OsMADS61 decreased root biomass, N accumulation and mycorrhization efficiency in its mutants. OsMADS61 could be directly regulated by another AM-upregulated OsMADS paralogue, OsMADS26, which itself can also activate OsNPF4.5, OsNRT2.2, and OsNAR2.1, encoding a nitrate transporter activating protein. Together, our results reveal a dual regulatory role for OsMADS61 and OsMADS26 in governing both direct and symbiotic nitrate uptake pathways.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE322nnn/GSE322741 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE322741 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1431394 ncbi.nlm.nih.gov/bioproject/PRJNA1431394 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing
- From keywords
- Life Sciences
Provenance · 1 source records, 6 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE322741 | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |