LCMS_Raw_Data_NPPM_Radiosensitization_Study

This dataset contains raw untargeted metabolomics data derived from human glioblastoma U87 MG cells, generated to investigate the radiosensitizing mechanism of biomimetic Fe3O4 nanoparticles (NPPM).Experimental Design and Sample Preparation:U87 MG cells were divided into four experimental groups: Negative Control (NC), NPPM Only (NP), Irradiation Only (IR), and NPPM combined with Irradiation (NP_IR). Cells in the NP and NP_IR groups were treated with NPPM (100 μmol/L).

After 24 hours of drug incubation, the medium was replaced, and the IR and NP_IR groups received a single dose of 5 Gy radiation (6 MV, 600 cGy/min). Cells were harvested 24 hours post-irradiation. Metabolites were extracted using a cold methanol/water (4:1, v/v) solution, followed by grinding, ultrasonication, and centrifugation to obtain the supernatant.Data Acquisition:The metabolic profiles were acquired using a Thermo Scientific Vanquish Horizon UHPLC system coupled with a Thermo Scientific Exploris 240 mass spectrometer.