Data · dataset · 2026
<p>Sph causes mitochondrial fragmentation, cristae disruption, mtDNA release, and cGAS-STING pathway activation.</p>
Listed in ZivaHub
<p><b>(A)</b> Representative transmission electron microscopy images showing mitochondrial ultrastructure in MAC-T cells infected with <i>S. aureus</i> WT, Δ<i>sph</i>, and Δ<i>sph</i>::<i>sph</i> (MOI = 200) for 6 h (n = 3).
Description
Original magnification, scale bars, 1 μm. <b>(B)</b> Representative confocal images showing cytosolic mtDNA release in MAC-T cells infected with the indicated <i>S. aureus</i> strains for 6 h (n = 3).
Scale bars, 10 μm. <b>(C)</b> Representative transmission electron microscopy images of mitochondrial ultrastructure in MAC-T cells treated with recombinant Sph (32 μg/mL) for 6 h (n = 3). Scale bars, 1 μm. <b>(D)</b> Representative confocal images showing mtDNA release in MAC-T cells treated with recombinant Sph (4 or 32 μg/mL) for 6 h (n = 3). Scale bars, 50 μm. <b>(E)</b> Intracellular ATP levels in MAC-T cells treated with recombinant Sph (4 or 32 μg/mL) for 6 h (n = 6). <b>(F)</b> Relative mRNA expression of the mitochondrial fusion genes <i>Mfn1</i> and <i>Mfn2</i> and the fission-related genes <i>Drp1</i> and <i>Fis1</i> in MAC-T cells following Sph treatment (4 or 32 μg/mL, 6 h) (n = 3).
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Violin plots illustrate the distribution of mRNA levels across the experimental groups. <b>(G)</b> Expression of POLG protein in MAC-T cells treated with treated with recombinant Sph (32 μg/mL), TMP (2,3,5,6-tetramethylpyrazine; 10 μM), or bortezomib (500 nM) for 6 h (n = 3). <b>(H)</b> Representative immunoblots and quantitative analysis of cGAS-STING pathway proteins in MAC-T cells treated with recombinant Sph (4 or 32 μg/mL) for 6 h (n = 3).
Data are presented as mean ± SD. *<i>P</i> < 0.05; **<i>P</i> < 0.01; ns, not significant.</p>
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Where it is published
- DOI doi.org/10.1371/journal.ppat.1014644.g004 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Biochemistry and cell biology · Earth & Environmental Science · Genetics · Immunology · Infectious diseases · Life Sciences · Medicine & Health · Microbiology · Research, science and technology policy · Virology
- Inferred from text
- Image 65% · Microscopy 75%
Provenance · 1 source records, 17 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/34026597 | 10 d ago | JSON v1 |
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| concepts[field].anzsrc:field:320211 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Infectious Diseases'] |
| concepts[field].anzsrc:field:440710 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Science Policy'] |
| concepts[field].anzsrc:group:3101 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Cell Biology'] |
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| concepts[field].local:field:life-sciences | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[modality].local:modality:image | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[modality].local:modality:microscopy | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/rights |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |