Multi-omics integrated analysis of the inhibitory mechanism of Tannic acid on MRSA biofilm

Biofilms markedly enhance bacterial resilience by impeding antibiotic penetration and evading host immune responses. Tannic acid (TA), a naturally occurring polyphenolic compound, exhibits broad-spectrum antimicrobial and anti-biofilm properties; however, the underlying mechanisms remain poorly understood.

In this study, the inhibitory effects of sub-inhibitory concentrations of TA on MRSA biofilm formation were systematically investigated through an integrative multi-omics approach (encompassing transcriptomic, proteomic, and metabolomic analyses) complemented by comprehensive phenotypic validation. Phenotypic assays demonstrated that TA effectively disrupted biofilm architecture and reduced biofilm biomass without exerting bacteriostatic or bactericidal effects.