Data Sheet 2_Cyclic stretch increases glucose uptake and pro-inflammatory response in rat alveolar epithelial type II cells.pdf

Non-stretched cells, stimulated or not with LPS, served as a control. Glucose oxidase activity was measured using a colorimetric/fluorometric assay, while glucose uptake was assessed by immunofluorescence using the fluorescent glucose analog 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxyglucose (2-NBDG). Gene expression and secretion were measured by real-time polymerase chain reaction (RT-qPCR) and enzyme-linked immunosorbent assay (ELISA), respectively.

Statistical analyses were performed using one-way ANOVA followed by Šidák’s multiple comparisons test, with significance set at p < 0.05.</p>Results<p>30% cyclic stretch significantly increased glucose uptake and glucose oxidase activity in both LPS-treated and untreated cells. Inflammatory gene expression and mediator secretion were predominantly increased in LPS-primed cells, although the effects varied according to the marker and time point.</p>Conclusions<p>Overall, excessive cyclic stretch increased the glucose-related readouts assessed in AECs L2 cells, whereas inflammatory responses were more strongly influenced by LPS stimulation and varied among mediators and time points.</p>