Development of analytical methodology for the detection of polar residues in food using LC-MS/MS

The method was validated in accordance with SANTE/11813/2017 guidelines.<br><br>The introduction of the UniSpray source further improved the sensitivity of the method compared to electrospray. This sensitivity gain allowed the simplification of the sample preparation procedure and the concentration step was removed. This method was fully validated in milk and milk powder according to the same validation guidelines. <br><br>Analytical strategies at different level were discussed across the optimisation of methods for the analysis of these three families of polar analytes.

The UniSpray ionization source was observed to generally improve the detector signal of polar compounds but can only be used in specific application conditions. HILIC is required for highly polar analytes although reversed-phase is more suitable for high-throughput analysis because of the shorter equilibration periods required. In this work, it was found that simplified extraction procedures were required to allow the combined analysis of both high polar with polar to non-polar molecules.<br><br><i>Thesis embargoed until 31st July 2028</i>