Data · dataset · 2025
Global gene expression profiles of cardiac progenitors differentiated from human pluripotent stem cells in 3D culture under simulated microgravity
Listed in NASA Data Portal
Efficient generation of cardiomyocytes from human pluripotent stem cells is critical for their regenerative applications.
Description
Microgravity and 3D culture can profoundly modulate cell proliferation and survival. Here we engineered microscale progenitor cardiac spheres from human pluripotent stem cells and exposed the spheres to simulated microgravity using a random positioning machine for 3 days during their differentiation to cardiomyocytes.
Methods
Read the rest (3 more)
RNA-seq libraries were prepared using the Illumina TruSeq RNA kit and the TrueSeq method was employed for mRNA enrichment. The libraries were quantified and samples were multiplexed in each lane of the flowcell. Cluster generation was performed and then sequenced on the Illumina HiSeq1000 system.
Reads were mapped on the Human Genome Reference and normalized expression table was generated. Results: Among differentially expressed genes 53 of them were up-regulated and 75 were down-regulated. Conclusions: Data demonstrate increased expression of genes associated with growth development and pro-survival in cardiac progenitors cultured under simulated microgravity compared with those cultured under standard gravity.
RNA-sequencing analysis was performed to compare global gene expression profiles of cells at differentiation day 8 under simulated microgravity vs. standard gravity.
Links
Get the data
- genelab-data.ndc.nasa.gov /genelab/accession/GLDS-127 ↗
Global gene expression profiles of cardiac progenitors differentiated from human pluripotent stem cells in 3D culture under simulated microgravity
download · from data nasa gov
Where it is published
- NASA Data Portal dataset page data.nasa.gov/dataset/global-gene-expression-profiles-of-cardiac-progenitors… ↗
landing page · from data nasa gov
Catalogue records · 1
- CKAN API data.nasa.gov/api/3/action/package_show?id=3ee899d3-18e0-4d55-987f-e8fdbdab2… ↗
metadata API · from data nasa gov
Topics
- From keywords
- Engineering
- Inferred from text
- Biomedical engineering 69% · Heart 65% · RNA sequencing 65% · Tabular 65%
Related
- Possibly the same asGlobal gene expression profiles of cardiac progenitors differentiated from human pluripotent stem cells in 3D culture under simulated microgravity
- Possibly the same asGlobal gene expression profiles of cardiac progenitors differentiated from human pluripotent stem cells in 3D culture under simulated microgravity
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NASA Data Portal | 3ee899d3-18e0-4d55-987f-e8fdbdab2af9 | 8 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[anatomy].local:anatomy:heart | enrichment · data nasa gov | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[field].anzsrc:group:4003 | enrichment · data nasa gov | taxonomy-embedding@1.1.0 | title+keywords+description (69%) |
| concepts[field].local:field:engineering | mapping · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| concepts[modality].local:modality:rna-seq | enrichment · data nasa gov | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[modality].local:modality:tabular | enrichment · data nasa gov | keyword-concept-rules@1.0.0 | title+description (65%) |
| created_date | source · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| description | source · data nasa gov | connector:data_nasa_gov@1.0.0 | /notes |
| license_text | source · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| publication_date | source · data nasa gov | connector:data_nasa_gov@1.0.0 | |
| title | source · data nasa gov | connector:data_nasa_gov@1.0.0 | /title |
| updated_date | source · data nasa gov | connector:data_nasa_gov@1.0.0 |