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Data · dataset · 2026

TB and SERS

Listed in Teesside University Research Data Repository

The plasma sample for this study was retrieved from our previous study (attached).

Description

In brief, a thousand of participants were recruited from community and hospital networks across eight provinces in northeastern Thailand. Of these, 500 cases were positive to interferon-gamma release assay (IGRA) or IGRA-positive and considered latent tuberculosis cases, while the other 500 individuals were IGRA-negative and served as healthy controls.

The participants included 258 males and 742 females, aged 5 to 99 years. The study was approved by the Khon Kaen University Ethics Committee (Ethics number HE651163). All experiments were performed in accordance with relevant guidelines and regulations.

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Protein was extracted from human plasma by published protocol. Then, the SERS measurements were conducted using a confocal Raman microscopic system (inVia Reflex confocal Raman microscope, Renishaw plc., UK). Each dried protein sample was individually mixed with sterile water to achieve a final concentration of 0.25 g/l.

Subsequently, 2.0 µl of protein sample was carefully placed onto the center of silver-based SERS sensors and allowed to air-dry for 10 min. A Raman spectrum was collected from a central region on each SERS sensor using a 785 nm wavelength laser through a 50× objective lens. Each spectral acquisition was performed with 25 mW laser power and a 10-second exposure time.

For each sample, spectral data were collected from 49 points covering a grid area of 7×7 at 15 µm steps.

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