Table · dataset · 2026
<p>Blocking ELISA inter-assay repeatability test.</p>
Listed in figshare and Loughborough Research Repository — shown once because both records carry DOI 10.1371/journal.pntd.0014755.t004
<div><p>Alveolar echinococcosis (AE) is a severe zoonotic parasitic disease caused by the larval stage of <i>Echinococcus multilocularis</i>, for which early and accurate diagnosis remains challenging.
Description
In this study, we targeted <i>E. multilocularis</i> thioredoxin peroxidase (EmTPx), a highly abundant excretory/secretory protein conserved across the genus <i>Echinococcus</i>, for diagnostic development. Monoclonal antibodies (mAbs) against EmTPx were generated, and two linear B‑cell epitopes, <sup>174</sup>HPGSK<sup>178</sup> and <sup>189</sup>SFMSS<sup>193</sup>, were identified.
Both epitopes are strictly conserved within <i>Echinococcus</i> but markedly divergent from mammalian host sequences. Structural modeling revealed that the epitopes are surface‑exposed, with <sup>174</sup>HPGSK<sup>178</sup> located in a flexible loop and <sup>189</sup>SFMSS<sup>193</sup> adjacent to an α‑helix, conformations favorable for antibody recognition. Two ELISA methods were established using the epitope‑specific mAbs.
Read the rest (2 more)
The blocking ELISA showed moderate diagnostic performance. More importantly, the sandwich ELISA enabled direct detection of EmTPx antigen in fecal samples of intermediate hosts, revealing a detection rate of 85% in mouse feces at an early infection stage. With a linear range of 0–250 ng/mL, a cutoff of 0.1724, and intra‑ and inter‑assay precision below 10%, this sandwich ELISA demonstrated robust analytical performance.
In conclusion, this proof-of-concept study identifies two evolutionarily conserved and structurally exposed EmTPx epitopes and demonstrates their potential utility for the development of monoclonal antibody-based approaches for non-invasive detection of echinococcosis.</p></div>
Links
Where it is published
- DOI doi.org/10.1371/journal.pntd.0014755.t004 ↗
DOI / persistent id · from figshare com
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from figshare com
Topics
- From keywords
- Astronomy & Astrophysics · Biochemistry and cell biology · Biochemistry and cell biology · Chemistry · Chemistry · Computer Science & AI · Computer Science & AI · Earth & Environmental Science · Earth & Environmental Science · Ecology · Ecology · Economics & Finance · Economics & Finance · Engineering · Engineering · Evolutionary biology · Evolutionary biology · Humanities · Humanities · Immunology · Immunology · Infectious diseases · Infectious diseases · Life Sciences · Life Sciences · Materials Science · Mathematics & Statistics · Medicine & Health · Medicine & Health · Microbiology · Microbiology · Ocean & Atmospheric Science · Psychology & Behavioral Science · Social Science · Social Science · Virology · Virology
- Inferred from text
- Disease 75%
Provenance · 2 source records, 43 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| figshare | oai:figshare.com:article/33968536 | 8 d ago | JSON v1 |
| Loughborough Research Repository | oai:figshare.com:article/33968536 | 8 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · figshare com | connector:figshare_com@1.0.0 | |
| concepts[disease].local:disease:disease | enrichment · figshare com | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].anzsrc:field:310706 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Virology'] |
| concepts[field].anzsrc:field:310706 | mapping · repository lboro ac uk | vocabulary-mapper@1.0.0 | keywords['Virology'] |
| concepts[field].anzsrc:field:320211 | mapping · repository lboro ac uk | vocabulary-mapper@1.0.0 | keywords['Infectious Diseases'] |
| concepts[field].anzsrc:field:320211 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Infectious Diseases'] |
| concepts[field].anzsrc:group:3101 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Cell Biology'] |
| concepts[field].anzsrc:group:3101 | mapping · repository lboro ac uk | vocabulary-mapper@1.0.0 | keywords['Cell Biology'] |
| concepts[field].anzsrc:group:3103 | mapping · repository lboro ac uk | vocabulary-mapper@1.0.0 | keywords['Ecology'] |
| concepts[field].anzsrc:group:3103 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Ecology'] |
| concepts[field].anzsrc:group:3104 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Evolutionary Biology'] |
| concepts[field].anzsrc:group:3104 | mapping · repository lboro ac uk | vocabulary-mapper@1.0.0 | keywords['Evolutionary Biology'] |
| concepts[field].anzsrc:group:3107 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Microbiology'] |
| concepts[field].anzsrc:group:3107 | mapping · repository lboro ac uk | vocabulary-mapper@1.0.0 | keywords['Microbiology'] |
| concepts[field].anzsrc:group:3204 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Immunology'] |
| concepts[field].anzsrc:group:3204 | mapping · repository lboro ac uk | vocabulary-mapper@1.0.0 | keywords['Immunology'] |
| concepts[field].local:field:astronomy | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:chemistry | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:chemistry | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:computer-science-ai | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:computer-science-ai | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:economics-finance | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:economics-finance | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:engineering | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:engineering | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:humanities | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:humanities | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:materials-science | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:mathematics-statistics | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:ocean-atmospheric | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:psychology-behavioral | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:social-science | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:social-science | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| description | source · figshare com | connector:figshare_com@1.0.0 | /metadata/dc/description |
| license | source · figshare com | connector:figshare_com@1.0.0 | /metadata/dc/rights |
| publication_date | source · figshare com | connector:figshare_com@1.0.0 | |
| title | source · figshare com | connector:figshare_com@1.0.0 | /metadata/dc/title |