Gene Expression Profiling of Synechococcus elongatus PCC 7942 and Rhodotorula toruloides NBRC 0880 (Rhodosporidium toruloides IFO0880) in Co-culture and Membrane-Separated Bioreactor Configurations Under Various Stress and Nutrient Conditions

Experiments included planktonic co-cultures in 50 mL tubes subjected to chemical stressors (oxidative stress, photosynthesis inhibition), temperature stress, nutrient limitation (nitrogen, phosphorus), pH extremes, and heterotrophic conditions, as well as physically separated co-cultures in a 600 mL membrane bioreactor.

Axenic controls for Synechococcus elongatus PCC 7942 were included for comparison. Differential gene expression analysis provides insight into interspecies interactions and individual organism responses across co-culture configurations.