12 datasets

NCBI GEO2026 · RNA sequencing · 3 samples

…Congenital heart disease (CHD) is a major global health problem. Although treatment has improved, children still face comorbidities that may shorten their life.… …Single-cell transcriptomics and functional assays revealed high activation states of T cells and higher granzyme and effector cytokine production in children with CHD.…

NCBI GEO2026 · RNA sequencing · 12 samples

…Single-nucleus RNA sequencing (snRNA-Seq) enables high-resolution transcriptomic profiling of complex tissues such as the heart, where dissociation into intact single cells is challenging… …Both platforms resolved major cardiac cell populations with consistent cell-type proportions and marker gene expression.…

NCBI GEO2026 · RNA sequencing · 3 samples

…Single-nucleus RNA sequencing (snRNA-seq) was performed on left ventricular tissue collected at Day 7 post-MI and treatment.… …This dataset provides a comprehensive single-nucleus transcriptional landscape of the post-infarct heart and enables cell type–specific analysis of transcriptional changes associated…

NCBI GEO2026 · RNA sequencing · 6 samples

…Background: CKM syndrome is characterized by pathophysiological interactions among the heart, the kidneys, and metabolic derangements.… …We leveraged a new translational model of CKM syndrome in aging swine and single-nucleus RNA sequencing (snRNA-seq) to investigate the renal cell-specific transcriptomic profile, thereby…

NCBI GEO2026 · RNA sequencing · 12 samples

…Recently, single-cell genomic approaches have revealed in unprecedented resolution the orchestrated cellular responses driving cardiac fibrosis.… …Yet, the fibrosis-causing phenotypes that emerge in the heart following non-ischemic cardiac stress, and the transcriptional circuits that govern cell identity and drive fibrosis, are…

NCBI GEO2026 · RNA sequencing · 14 samples

…Our study directly addresses this critical knowledge gap by performing an integrative multi-omics study—combining single-nucleus RNA/ATAC sequencing and high-resolution spatial transcriptomics… …(MERFISH) on human EFE and healthy heart tissues.…

NCBI GEO2026 · Non-coding RNA profiling by array · 18 samples

…Methods: Using comprehensive single cell and spatial transcriptomic analyses, we characterized the cellular composition and gene expression in preserved, granulomatous, and fibrotic… …regions of human CS hearts.…

NCBI GEO2026 · Raster · GeoTIFF · 6 samples

…Nine sections (one per patient, 3 per group; 625,305 cells) were profiled with a 477-gene panel.… …Part of a multimodal atlas that also includes bulk RNA-seq, single-nucleus RNA-seq and a murine pulmonary artery banding model.…

NCBI GEO2026 · RNA sequencing · 41 samples

…METHODS:Using comprehensive single-cell and spatial transcriptomic analyses, we characterized the cellular composition and gene expression in preserved, granulomatous, and fibrotic… …regions of human CS hearts.…

NCBI GEO2026 · Image data · 9 samples

…Stem cell-derived organoids hold promise to model tissue-specific disease.… …This study presents the first systematic profiling of multiple stem cell derived organoid models (brain, heart muscle, heart valve, kidney, lung, cartilage, and haematopoietic) with…

NCBI GEO2026 · RNA sequencing · 56 samples

…Cardiac regeneration is limited in the adult mammalian heart due to restricted cardiomyocyte proliferation and persistent fibrosis.… …Here, integrated single-nucleus RNA-seq and ATAC-seq reveal multicellular transcriptional and epigenetic reprogramming underlying this response.…

NCBI GEO2026 · RNA sequencing · 8 samples

…However, the characteristics and functions of the senescent cells (Sen.) following MI-induced cardiac remodeling remains elusive.… …Then we analyzed our single-nucleus (sn)-multiome and fluorescence-based (SPiDER-β-gal/p16-EGFP) spatial transcriptomics data from the infarcted heart on day 7 post-MI to identify the…