Omics · study · 2026
Gene expression profiles at the single-nucleus level in heart tissue after myocardial infarction and saNppa-LNP treatment
Listed in NCBI GEO
This project aims to investigate the cellular and molecular mechanisms underlying the therapeutic effects of a self-amplifying RNA encoding atrial natriuretic peptide (saNppa) in myocardial infarction (MI).
Description
In this study, a myocardial infarction model was established in C57BL/6 mice by coronary artery ligation. On the day of surgery, mice received intramuscular injection of saNppa formulated in lipid nanoparticles (saNppa-LNP) or phosphate-buffered saline (PBS) as control.
Single-nucleus RNA sequencing (snRNA-seq) was performed on left ventricular tissue collected at Day 7 post-MI and treatment. Three experimental groups were included: sham-operated mice, MI mice treated with PBS (MI+PBS), and MI mice treated with saNppa-LNP (MI+saNppa-LNP). This dataset provides a comprehensive single-nucleus transcriptional landscape of the post-infarct heart and enables cell type–specific analysis of transcriptional changes associated with myocardial infarction and saNppa self-amplifying RNA therapy.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE313nnn/GSE313956 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE313956 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1387437 ncbi.nlm.nih.gov/bioproject/PRJNA1387437 ↗
project · from NCBI GEO
- PubMed 41785353 pubmed.ncbi.nlm.nih.gov/41785353 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Heart 75% · Myocardial infarction 75% · RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 65%
Provenance · 1 source records, 12 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE313956 | 9 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[anatomy].local:anatomy:heart | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[disease].local:disease:myocardial-infarction | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |