Table 1_DNA damage repair-related gene signatures delineate immune-distinct subtypes and diagnostic cues in tuberculosis.xlsx

Background<p>DNA damage repair-related genes (DDR-RGs) may shape tuberculosis (TB) pathogenesis, but their molecular characteristics, diagnostic utility, and cellular context remain insufficiently defined.</p>Methods<p>We integrated multiple GEO transcriptome datasets, identified differentially expressed genes, constructed weighted gene co-expression network analysis modules, and intersected these results with curated DDR-RGs to obtain candidate genes.

An ensemble framework of 110 machine-learning model combinations was used to select diagnostic markers, followed by external validation, consensus clustering, immune infiltration analysis, single-cell RNA-seq evaluation, and Comparative Toxicogenomics Database-based compound-association analysis.</p>Results<p>The integrated training cohort included 134 samples; 94 differentially expressed genes and a 392-gene key module were identified, yielding 25 candidate DDR-RGs.