Omics · study · 2026
Stem Cells in Muscle Contractures of Children with Cerebral Palsy have Decreased Myogenic and Increased Fibrotic Transcripts
Listed in NCBI GEO
Development of muscle contractures are common in Cerebral palsy (CP) and characterized by high muscle stiffness that limits function and mobility.
Description
The state of the cell types within muscle, particularly muscle stem cells (MuSCs) and fibro-adipogenic progenitors (FAPs), are largely unknown. The aim of this study was to leverage single cell RNA sequencing technology to determine how specific cell types are altered in contracture.
CP MuSCs demonstrated significant upregulation of fibrotic genes (ACTA2) and down regulation of myogenic genes (MYF5) compared to typically developing MuSCs. The most dramatically up-regulated genes in FAPs in CP were also major fibrotic components (COL1A1). Interacting signals between FAPs, MuSCs, and immune cells, such as TNF signaling between macrophages and FAPs, were identified that support contracture progression.
Read the rest (1 more)
The study reveals specific genes and signals as therapeutic targets to reduce muscle contracture in children with CP.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE309nnn/GSE309114 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE309114 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1333979 ncbi.nlm.nih.gov/bioproject/PRJNA1333979 ↗
project · from NCBI GEO
- PubMed 42021719 pubmed.ncbi.nlm.nih.gov/42021719 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE309114 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |