Single-Cell Transcriptomics of Nfe2l2-KO versus WT Mouse Corneas

Library preparation and sequencing were outsourced to Singleron Biotechnologies (Nanjing, China). Raw reads were aligned to mm10 with STAR/Drop-seq Tools; ambient RNA was corrected with SoupX.

In Seurat v5, low/high feature outliers and high-mitochondrial cells were removed, doublets were filtered (DoubletFinder), SCTransform normalization and integration were applied, clusters were identified by SNN/UMAP, and annotated with canonical markers. Differential expression used Wilcoxon with BH FDR < 0.05. Functional enrichment of DEGs was performed with Metascape.