Transcriptomic profiling of astrocytes under three extracellular acidification conditions

Astrocytes are central regulators of brain homeostasis and are highly responsive to changes in extracellular pH. However, transcriptomic resources comparing different experimentally induced acidotic conditions in astrocytes remain limited.

Here, we generated an RNA sequencing dataset for the mouse astrocyte cell line C8-D1A exposed to three extracellular acidification-related conditions, including lactic acid treatment, 15% CO2 exposure, and NaHCO3-low medium, together with untreated controls. This dataset captures both shared and condition-specific transcriptomic responses of astrocytes under distinct extracellular acidification paradigms. Functional enrichment analyses highlighted biological pathways related to stress response, metabolism, inflammatory signaling, and cellular adaptation to extracellular acidification.