Single Cell Smart-Seq 3 RNA-Seq and Bulk Exome Seq from Breast Cancer Patients

The dataset contains raw sequencing data for various high-throughput molecular tests performed on two sample types: tumor samples from two breast cancer patients and cell lines derived from High-grade serous carcinoma Patients.

The breast cancer data comes from two patients: patient 1 (BCSA1) has two tumor regions A-B and patient 2 (BCSA2) has five regions(A-E). For a normal sample and each region from each patient Whole Exome Sequencing was performed using Twist Biosciences Human Exome Kit by the SNP&SEQ Technology platform, SciLifeLab, National Genomics Infrastructure Uppsala, Sweden. Also for each patient, EPCAM+ CD45- sorted cells from all the regions where sorted to a 384 well plate, and Smart-Seq3 libraries were prepared at Karolinska Institutet and sequenced at National Genomics Infrastructure Uppsala, Sweden.