Omics · study · 2026
Galaxamide improve cisplatin-induced uterine damage via attenuating inflammation and apoptosis in in mice
Listed in NCBI GEO
Objective: To evaluate the protective effects of marine cyclopeptide galaxamide against cisplatin-induced uterine toxicity in cervical cancer models, focusing on macrophage polarization and NF-κB signaling modulation
Description
Methods
In vivo tumor-bearing mouse models and in vitro cervical cancer cell lines were treated with cisplatin (CIS, 5 mg/kg) ± galaxamide (20 mg/kg). Uterine histopathology (hematoxylin-eosin staining), apoptosis (TUNEL assay), macrophage subsets (flow cytometry for M1/M2 markers), NF-κB activation (western blot for p65 phosphorylation), and serum cytokines (ELISA for TNF-α/IL-6) were analyzed. Embryo implantation rates were assessed to quantify endometrial receptivity Results: Cisplatin caused significant endometrial atrophy (↓45% thickness vs. control, P<0.01), NF-κB hyperactivation (2.8-fold p65 increase), and M1-macrophage dominance (M1/M2 ratio ↑40%).
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These changes correlated with elevated pro-inflammatory cytokines (TNF-α↑300%, IL-6↑180%) and reduced embryo implantation (↓67%, P<0.001). Galaxamide co-treatment: Suppressed NF-κB activation (p65↓65%, P<0.01) and apoptosis (TUNEL+ cells↓50%) Restored M2-macrophages (↑25%, P<0.05) and cytokine balance (TNF-α↓55%, IL-6↓48%) Preserved endometrial integrity and increased implantation rates (1.9-fold vs. CIS alone, P<0.01) Conclusion: Galaxamide mitigates cisplatin-induced uterine damage by reprogramming macrophages toward M2 phenotype and inhibiting NF-κB-mediated inflammation, offering a promising adjuvant strategy to preserve fertility in young cervical cancer patients Keywords: cisplatin toxicity, uterine protection, galaxamide, macrophage polarization, NF-κB pathway
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE302nnn/GSE302127 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE302127 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1289027 ncbi.nlm.nih.gov/bioproject/PRJNA1289027 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Cancer 75%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE302127 | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |