Xenium single cell spatial transcriptomics from patient-derived glioblastoma organoid cultured on brain slice and a primary glioblastoma sample

Here, we performed probe-based Xenium single-cell spatial transcriptomics on a primary human glioblastoma sample and on a patient-derived glioblastoma organoid cultured on a brain slice. The aim was to assess the in situ single-cell expression of hypoxia-related genes, with a particular focus on VEGFA and CXCL12.

These datasets provide spatially resolved transcriptomic information to study hypoxic niches and tumor microenvironmental interactions in glioblastoma.