Excel · study · 2026
Transcriptomic assessment of host responses in Vaccinia and Venezuelan equine encephalitis virus-infected human dendritic cells
Listed in NCBI GEO
Understanding host cell response to viral infection could lead to the identification of molecular targets that can be used for the development of diagnostics and therapeutics.
Description
In this study, we investigated human dendritic cell (DC) response to infections with Vaccinia (VAC) virus, a highly immunogenic poxvirus and Venezuelan Equine Encephalitis (VEEV) virus, a single-stranded positive strand RNA Alphavirus using human gene expression (mRNA) microarrays.
Comparative changes in DC mRNA expression resulting from infection by the two viruses at 1-, 8-, and 12-hours post-infection (hpi) were determined. Most changes in mRNA expression occurred during 8-12 hpi. Expression of genes involved in the proinflammatory, blood circulation and regulation of innate immune response pathways was more pronounced in VEEV-infected cells than VAC-infected-cells.
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VAC-infected cells showed selective expression of genes involved in platelet activation, DNA damage and macromolecule synthesis pathways. mRNA expression from both exposures showed stronger and immediate response in VAC infection when compared to uninfected controls whereas delayed response was observed in VEE. This study identifies a broad range of pathways affected by VAC and VEE, highlighting the complex interplay between immune activation, cellular stress, and gene regulation.
The specific up- or down-regulation of these pathways at different time points likely dictates the overall outcome of the viral infection and could potentially lead to improved pre-symptomatic surveillance and diagnosis.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE309nnn/GSE309062 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE309062 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1333882 ncbi.nlm.nih.gov/bioproject/PRJNA1333882 ↗
project · from NCBI GEO
- PubMed 42072665 pubmed.ncbi.nlm.nih.gov/42072665 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by array · Homo sapiens
- From keywords
- Life Sciences
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE309062 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-array | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |