Spatial and single-nucleus multi-omics profiling reveals heterogeneous endothelial cell overabundance in endocardial fibroelastosis [MERFISH]

The absence of effective therapies for EFE stems from a fundamental lack of understanding of its underlying cellular and molecular mechanisms. Progress in investigating EFE pathogenesis has been further constrained by the scarcity of patient-derived samples.

Our study directly addresses this critical knowledge gap by performing an integrative multi-omics study—combining single-nucleus RNA/ATAC sequencing and high-resolution spatial transcriptomics (MERFISH) on human EFE and healthy heart tissues.