ChIP-seq profiling of MNDA in iPSC-derived human Sertoli-like cells

To explore the genomic binding and regulatory capacity of MNDA in Sertoli cells, we used human induced pluripotent stem cell-derived Sertoli-like cells (hiSCs) as an in vitro human Sertoli cell model, whose preparation procedures were adapted from the previously reported study (Liang et al., eLife, 2019). MNDA expression was induced in hiSCs via IFNα stimulation, and ChIP-seq was conducted to capture genome-wide MNDA binding events.

Bioinformatic analysis identified thousands of specific MNDA binding peaks throughout the genome. These peaks were distributed across diverse genomic regions, including intergenic regions, gene bodies and regulatory regions flanking transcription start sites. Collectively, these results indicated that MNDA binds to multiple types of genomic loci and may modulate the transcription of downstream target genes.