Archive · study · 2026
MERFISH data on seven mouse brain with Abeta pathology
Listed in NCBI GEO
Microglia adopt various spatially resolved gene and functional profiles in amyloidosis mice, for example the relative enrichment of DAMs proximal to plaques.
Description
To spatially resolve these transcriptional changes and identify microglial populations responsive to TREM2 small molecule agonism, multiplexed error-robust fluorescence in situ hybridization (MERFISH) was conducted on sagittal brain sections from VG-3909–treated 5xFAD;hTREM2 mice, 12 hours post-oral dosing.
Simultaneous immunostaining using an anti-amyloid antibody enabled spatial correlation of microglial transcriptomes with amyloid plaque localization. The custom MERFISH panel comprised 500 genes, included cell-type markers and microglia-focused genes.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE327nnn/GSE327147 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE327147 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1449569 ncbi.nlm.nih.gov/bioproject/PRJNA1449569 ↗
project · from NCBI GEO
Topics
- Stated by source
- Mus musculus · Other
- From keywords
- Life Sciences
- Inferred from text
- Genetics 70%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE327147 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].anzsrc:group:3105 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (70%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:other | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |