Data Sheet 1_Enrichment of the TRIM21-IgG complex in placental villi during HIV-1 infection.docx

However, the dynamics of placental immunity in pregnant women with chronic HIV-1 infection undergoing suppressive antiretroviral therapy (ART) remain poorly understood. Tripartite motif (TRIM) family proteins act as critical host restriction factors or indirect regulators of antiviral pathways through ubiquitination cascades. </p>Objectives <p>In this study, we profiled the transcriptional expression of prominent antiviral TRIM genes − namely TRIM5α, TRIM8, TRIM21, TRIM22, TRIM39, TRIM64B, and TRIM71 − in both the decidual and chorionic villi compartments of HIV-1-infected parturient women with undetectable viral loads compared to healthy controls (HC). </p>Methods<p>Quantitative real-time PCR in the decidua and villi was performed in both the decidual and chorionic villi compartments of HIV-1-infected parturient women compared to HC.

Gene network was generated using GeneMANIA was assessed to identify functionally related genes. Given that TRIM21 classically intercepts antibody-coated target complexes, we evaluated its spatial intersection with maternal humoral components via immunofluorescence using a TissueGnostics scanning confocal microscope. </p>Results<p>Quantitative real-time PCR revealed compartment-specific alterations, with a significant upregulation of TRIM21, TRIM39, and TRIM64B in the decidua, and TRIM21, and TRIM39 in the chorionic villi of the HIV-1 group.