Omics · study · 2026
Single Cell Profiling of Human Trabecular Meshwork Cells
Listed in NCBI GEO
Elevated intraocular pressure (IOP) is currently the sole modifiable risk factor for glaucoma, a leading cause of irreversible blindness worldwide.
Description
The trabecular meshwork (TMW) plays a pivotal role in regulating IOP by facilitating aqueous humor outflow. We performed single cell expression quantitative trait loci (eQTL) mapping in TMW cells collected from 74 people (45 males, 29 females) donors.
Following isolation and culture with or without dexamethosone, a total of 316,799 singlet cells were captured and underwent RNA sequencing. Differential gene expression analysis revealed 63 upregulated (log2 fold change >3) and 17 downregulated (log2 fold change <3) genes following dexamethasone exposure. Understanding the genetic underpinnings of IOP regulation through an improved understanding of the molecular pathways involved in TMW homeostasis will facilitate novel therapeutic development.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE313nnn/GSE313736 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE313736 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1381509 ncbi.nlm.nih.gov/bioproject/PRJNA1381509 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE313736 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |