Omics · study · 2026
RNA-seq of placentas from FNDC5-deficient and wild-type mice at gestational day 17.5
Listed in NCBI GEO
Fibronectin type III domain–containing protein 5 (FNDC5), the precursor of the myokine irisin, has been implicated in metabolic regulation and pregnancy outcomes, but its role in placental development is not well understood.
Description
To investigate how FNDC5 deficiency affects the placental transcriptome, we performed mRNA sequencing on placentas collected at gestational day 17.5 from FNDC5 knockout (FNDC5-/-) and wild-type mice.
Total RNA was isolated from whole placentas, poly(A)+-selected, and used to generate strand-specific libraries with the NEBNext Ultra II Directional RNA Library Prep Kit, followed by paired-end sequencing on an Illumina NovaSeq 6000. These data enable identification of FNDC5-dependent changes in placental gene expression associated with alterations in fetal–placental growth and maternal body weight.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE313nnn/GSE313842 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE313842 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1381659 ncbi.nlm.nih.gov/bioproject/PRJNA1381659 ↗
project · from NCBI GEO
- PubMed 42185335 pubmed.ncbi.nlm.nih.gov/42185335 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 65% · Sequencing 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE313842 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |