Excel · study · 2026
The MIME-seq technique allows to monitor the interaction of small non-coding RNAs with Argonaute proteins and their transfer to other cells
Listed in NCBI GEO
The MIME-seq2.0 technique, developed by Mandlbauer et al., utilizes a chimeric HEN1 methyltransferase engineered to bind to Argonaute proteins (MIME enzyme) to protect the terminal sugar of small non-coding RNAs (sncRNAs) from oxidation, a reaction that inhibits RNA ligation and cloning.
Description
In this study, we confirm that the MIME-seq technique efficiently distinguish oxidized and non-oxidized sncRNAs using RNA sequencing and quantitative PCR.
Upon transfection of the insulin-secreting cell line MIN6B1 with or without the MIME enzyme, we observed a significant loss in the detection of miRNAs following RNA oxidation, with a strong recovery rate in MIME-expressing cells. Comparison of the sncRNA profile in wild type and MIME-expressing cells demonstrated protection from oxidation not only of miRNAs, but also of some Y-RNA and tRNA-derived fragments. Immunoprecipitation with Ago2 antibodies confirmed that the protected Y-RNA and tRNA fragments bind to Argonaute proteins.
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We also used this system to track miRNA transfer between cells via extracellular vesicles (EVs). miRNAs released in EVs of MIME-expressing Jurkat T cells or C2C12 myotubes and delivered to MIN6B1 cells were protected from oxidation, enabling sensitive and specific detection of the transferred miRNAs in the receiving cells. Overall, the MIME-seq technique provides a powerful tool for analyzing RNA methylation and Argonaute binding in living cells and offers new possibilities for tracking RNA transfer across different cell types.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE313nnn/GSE313744 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE313744 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1381533 ncbi.nlm.nih.gov/bioproject/PRJNA1381533 ↗
project · from NCBI GEO
- PubMed 41844788 pubmed.ncbi.nlm.nih.gov/41844788 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Mus musculus · Non-coding RNA profiling by high throughput sequencing
- From keywords
- Life Sciences
- Inferred from text
- Agricultural biotechnology 71% · RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE313744 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].anzsrc:group:3001 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (71%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:non-coding-rna-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |