Omics · study · 2026
PRDM16 Modulates Aspects of Cell-Cycle Dynamics and Maturation in Human iPSC-Derived Cardiomyocytes
Listed in NCBI GEO
Understanding the molecular mechanisms that mediate entry into quiescent states of cardiac tissues, along with their associated metabolic rewiring, is crucial for comprehending the postnatal changes driving physiological hypertrophy and functional maturation.
Description
PRDM16 has been identified as a critical regulator of both proliferation and maturation in fetal-like human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs).
Our study demonstrates that PRDM16 orchestrates the proliferative axis of hiPSC-CMs by modulating CDK1 and phospho-AKT levels, two key drivers of cardiac proliferation. Reduced PRDM16 expression significantly impairs the metabolic and structural maturation of hiPSC-CMs, leading to dysregulated sarcomeric protein expression ratios and compromised engineered heart tissue (EHT) function. Conversely, mild PRDM16 overexpression promotes metabolic shifts and structural protein changes toward maturation.
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These findings provide new insights into the molecular mechanisms governing cardiomyocyte development and highlight PRDM16 as a potential therapeutic target for enhancing cardiac regeneration and repair, since transient PRDM16 downregulation creates a roadblock to maturation in favor of proliferation. Overall, this work underscores the central role of PRDM16 in controlling the proliferation-maturation axis of cardiomyocyte biology.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE307nnn/GSE307782 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE307782 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1327318 ncbi.nlm.nih.gov/bioproject/PRJNA1327318 ↗
project · from NCBI GEO
- PubMed 42425086 pubmed.ncbi.nlm.nih.gov/42425086 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Biochemistry and cell biology 69% · Heart 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE307782 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[anatomy].local:anatomy:heart | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].anzsrc:group:3101 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (69%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |