Markers for mitotic catastrophe in sodium valproate-treated HeLa cells

These proteins are ascribed to be potentially associated with the incidence of mitotic catastrophe, an oncosupressive event. Cells were fixed in methanol at -20 °C for 10 min, rinsed in PBS, permeabilized with 0.2% Triton X-100 for 10 min, and blocked in 5% BSA for 1 h.

Rabbit anti-caspase-2 and anti-p53 were used as primary antibodies (1:200 and 1:100 dilutions, respectively). Goat anti-rabbit conjugated with Alexa-Fluor 488 (1:500 dilution) was used as secondary antibody. The images were captured in a Leica TCS SP5 II confocal microscope at the Laboratório Central de Tecnologias de Alto Desempenho em Ciências da Vida (LaCTAD/UNICAMP).