Omics · study · 2026
Housing Mice in Thermoneutrality Causes Tissue-specific Changes in Number, Identity, and Phase of Circadian-expressed mRNA Transcripts
Listed in NCBI GEO
Mice housed at room temperature (RT, 25°C) experience chronic mild cold stress compared with those housed at thermoneutrality (TN, 30°C).
Description
We hypothesized that cold stress suppresses circadian transcript expression in peripheral tissues. RNA-seq of hearts, livers, and diaphragms collected every 4 hours over 48 hours in constant darkness identified mRNA transcripts exhibiting ≈24-hour rhythms (REGs).
TN produced tissue-specific changes in REG number, identity, and phase without altering core circadian clock transcript levels. Cardiac REGs increased 4-fold, diaphragm REGs 1.5-fold, and hepatic REG identity shifted substantially. GO analysis revealed coordinated reorganization of rhythmic metabolic programs in the heart and liver.
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These data demonstrate that ambient housing temperature has tissue-specific effects on the number, identity, and temporal organization of rhythmically expressed transcripts in the heart, liver, and diaphragm.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE337nnn/GSE337330 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE337330 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1484861 ncbi.nlm.nih.gov/bioproject/PRJNA1484861 ↗
project · from NCBI GEO
- PubMed 42146544 pubmed.ncbi.nlm.nih.gov/42146544 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Heart 75% · RNA sequencing 65%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE337330 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[anatomy].local:anatomy:heart | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |