Omics · study · 2026
Combinatorial E2F knockdown in the prostate cancer PM154 cell line
Listed in NCBI GEO
Treatment-emergent neuroendocrine prostate cancer (CRPC-NE) arises through RB1 loss and lineage plasticity in response to androgen receptor-targeted therapies, creating constitutive E2F transcription factor addiction that lacks effective therapeutic exploitation.
Description
All six E2F family members harbour a conserved U12-type minor intron within their dimerisation domain — processed exclusively by the minor spliceosome — creating a single post-transcriptional node whose inhibition simultaneously targets the entire activator E2F programme.
Combinatorial E2F knockdown RNA-seq revealed E2F3 as the dominant activator E2F and uncovered a paradoxical dual role for the E2F family as gatekeepers of neuroendocrine identity — complete E2F depletion unleashing an ASCL1-driven differentiation programme resembling pancreatic beta cell specification.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE346nnn/GSE346902 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE346902 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1526794 ncbi.nlm.nih.gov/bioproject/PRJNA1526794 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Cancer 75% · RNA sequencing 65%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE346902 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |