Cell-intrinsic functions of PD-L1 activate autophagy and prevent cytokine-induced apoptosis

We hypothesized that target tissue specific mechanisms also play a role and studied these mechanisms in thyrocytes. We discovered that thyroidal PD-L1 acts as a cytoprotective regulator, promoting cell survival during cytokine-induced stress.

PD-L1 suppression in thyrocytes amplified IFNγ-driven stress by aberrantly activating AKT - ERK - mTORC1, inhibiting autophagy, augmenting cellular stress, and triggering apoptosis. In a mouse model of ICI-thyroiditis, anti-PD-L1 treatment triggered immune activation while promoting thyrocyte apoptosis. Mechanistically, in mice, both pharmacologic and genetic downregulation of thyroidal PD-L1 caused autophagic defects.