Omics · study · 2026
SMART-seq profiling of the ascending aorta and aortic arch from male and female Wnt1-Cre;Rosa26-mTmG mice
Listed in NCBI GEO
Vascular smooth muscle cells (VSMCs) in the ascending aorta and aortic arch arise from two distinct embryonic origins: the second heart field (SHF) and the cardiac neural crest (CNC).
Description
Whether these populations exhibit sex-dependent differences remains unknown. Here, we used Wnt1-Cre;Rosa26-mTmG mice to genetically label CNC-derived VSMCs with GFP, while non-CNC-derived cells expressed tdTomato.
The ascending aorta and aortic arch were dissected from male and female mice, and single-cell suspensions were generated by enzymatic digestion. Cells were then sorted by fluorescence-activated cell sorting (FACS) based on GFP or tdTomato expression. RNA-expression profiles for each group were obtained using the Smart-seq platform.
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Thi study revealed the differential expression of VSMCs from different embryonic origin in both sexes.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE329nnn/GSE329247 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE329247 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1458202 ncbi.nlm.nih.gov/bioproject/PRJNA1458202 ↗
project · from NCBI GEO
- PubMed 42069076 pubmed.ncbi.nlm.nih.gov/42069076 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Heart 75%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE329247 | 6 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[anatomy].local:anatomy:heart | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |