NET1 mRNA localization to the midbody is required for ARP2/3-dependent initiation of mitotic abscission

We find that RNA localization and local translation at the midbody regulates when and where abscission-regulating proteins are expressed. The 3′UTR of NET1 mRNA contains an element that is necessary and sufficient for RNA targeting to the midbody.

Mislocalization of NET1 mRNA results in a loss of NET1 protein, a Rho family GEF, throughout the intercellular bridge as well as slower cell proliferation and delayed abscission. This coincides with a loss of Arp2/3 at the abscission site and is dependent upon NET1 binding to Rho family GTPases. These findings establish midbody RNA localization and local translation as a key layer of regulation over abscission timing and identify a role for NET1 as a regulator of Arp2/3-mediated branched actin accumulation at the abscission site.