Omics · study · 2026
Effect of PPARγ2 acetylation in at lysine 382 in mouse beige adipocyte differentiation
Listed in NCBI GEO
PPARr is a master regulator of adipogenesis which undergo different post translational modification during adipogenesis.
Description
This post translational modification of PPARγ control unique subset of genes rather full PPARr target genes. Recently we reported that SIRT7 deacetylate PPARr2 at lysine 382 residue and regulate lipogenesis in C3H10T1/2 cells.
Here, we investigate this SIRT7-mediated PPARγ2 lysine deacetylation in mouse beige adipocyte differentiation of subcutaneous white AT (scWAT) stromal vascular fraction (SVF) cell line. At first we prepared PPARr2 K382 acetylation (KQ) and deacetylation (KR) mimicking mutant expressing mouse scWAT SVF cell line. After beige adipocyte differentiation, we isolated total RNA at day 2 and performed RNA-seq analysis.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE317nnn/GSE317859 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE317859 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1415722 ncbi.nlm.nih.gov/bioproject/PRJNA1415722 ↗
project · from NCBI GEO
- PubMed 42274620 pubmed.ncbi.nlm.nih.gov/42274620 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 65%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE317859 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |