Data · study · 2026
SerRS mediates translational readthrough of through engagement of the selenocysteine incorporation machinery
Listed in NCBI GEO
Translational readthrough of UGA stop codons by selenocysteine-specific tRNAs (tRNASec) provide the basis for selenoprotein biosynthesis.
Description
Seryl-tRNA synthetase (SerRS) charges tRNASec with serine, which is modified into selenocysteine, and delivered to the ribosome by a designated elongation factor (eEFSec). We found that the selenocysteine incorporation machinery, including SerRS, tRNASec, and eEFSec, also mediated translational readthrough of non-selenocysteine genes, including VEGFA, to create C-terminally extended protein isoforms.
SerRS bound a subset of non-selenocysteine mRNAs to facilitate serine insertion. SerRS-mediated readthrough is sufficient to reverse premature termination caused by a disease-causing nonsense mutation. Genes involved in hypoxia, angiogenesis, and adhesion are enriched among SerRS-bound genes.
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This regulatory function of SerRS in mRNA translation is dependent on its enzymatic activity but also a vertebrate-specific domain involved in non-tRNA nucleic acid interaction. Our findings offer an avenue for therapeutic targeting of nonsense mutations through regulation of existing protein synthesis machinery exclusively using unmodified factors.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE206nnn/GSE206674 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE206674 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA851750 ncbi.nlm.nih.gov/bioproject/PRJNA851750 ↗
project · from NCBI GEO
- PubMed 37739431 pubmed.ncbi.nlm.nih.gov/37739431 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Homo sapiens · Other
- From keywords
- Life Sciences
- Inferred from text
- Biochemistry and cell biology 68%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE206674 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].anzsrc:group:3101 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (68%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:other | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |