Omics · study · 2026
Conditional overexpression of PPARα in intestinal epithelium diminishes GIP enteroendocrine cells and circulating hormone levels
Listed in NCBI GEO
PPARα transcriptional programs have tissue-dependent features, yet there is a lack of genetic research tools to study tissue-specific signaling activation without the addition of a systemic agonist.
Description
Our aim was to investigate intestinal epithelial-specific roles of PPARα signaling using a novel transgenic mouse that enables spatial and temporal control of Ppara overexpression. CAG-Ppara,-EGFP mice were bred to Villin-CreERT2 to establish the IEC-Ppara mouse, which was compared to littermate controls 2 weeks after tamoxifen exposure.
Transcriptional analysis of intestinal tissue from IEC-Ppara mice showed upregulation of PPARα target genes and functional enrichment for fatty acid catabolic processes. Unexpectedly, the enteroendocrine hormone Gip was among the most downregulated genes. GIP-positive enteroendocrine cell number, GIP circulating hormone, and the incretin response were also reduced in IEC-Ppara mice.
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These findings reveal PPARα as a regulator of GIP and support a new framework in which PPARα signaling influences glucose homeostasis via a gut hormone axis
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE336nnn/GSE336031 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE336031 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1480602 ncbi.nlm.nih.gov/bioproject/PRJNA1480602 ↗
project · from NCBI GEO
- PubMed 42595008 pubmed.ncbi.nlm.nih.gov/42595008 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE336031 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |