Transcriptome analysis of effects of TPH1 in prostate cancer cell lines

Elevated TPH1 expression is observed in NEPC cell lines and patient tumor samples. To elucidate the role of TPH1 in prostate cancer development and progression, we performed RNA sequencing (RNA-seq) in prostate cancer cells with either TPH1 knockdown or overexpression, compared to respective control cells.

Differential gene expression analysis and subsequent pathway enrichment revealed key molecular programs regulated by TPH1. Functionally, TPH1 overexpression promotes tumor cell proliferation and the acquisition of neuroendocrine features, while genetic or pharmacological inhibition of TPH1 suppresses NEPC cell growth and neuroendocrine marker expression. These findings underscore the oncogenic role of TPH1 in NEPC and support its potential as a therapeutic target.