Omics · study · 2026
pH-Responsive Nanostructured Calcium Phosphate Microrods as Pulmonary Delivery Platform: Fabrication, Characterization, and Comparative Assessment of Cytotoxic and Transcriptomic Responses in Alveolar Macrophages
Listed in NCBI GEO
Nanostructured, rod-shaped microparticles represent a promising drug-delivery plat-form for pulmonary delivery and targeting of alveolar macrophage by leveraging the aerodynamic advantages of fiber-like geometries.
Description
These microrods feature a hierar-chical architecture, designed for potential macromolecular payloads, and silica-based systems have previously been shown to successfully deliver oligonucleotides in vitro. However, current microrod systems mainly rely on nanoparticulate silica-based frameworks with limited biodegradibility and lack targeted disintegration under the acidic conditions encountered in phagolysosomes.
Therefore, a nanostructured calcium phosphate framework is proposed as a biodegradable and resorbable alternative with pH-responsive characteristics. This study describes the fabrication of nanostructured, rod-shaped calcium phosphate microparticles and discusses their suitability as a po-tential pulmonary drug-delivery platform. The particles feature a dissolution-driven disintegration in acidic and ion-rich environments relevant to phagolysosomes.
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In ad-dition, the particles exhibited a favorable acute cytotoxicity profile in the murine alve-olar macrophage cell line MH-S compared with their SiO2-based counterpart. Com-parative RNA-seq analysis of MH-S that are exposed to the particles indicate a mild transcriptomic response, while canonical signatures of classical or alternative macro-phage activation programs were not observed, supporting a generally well-tolerated exposure profile of the carrier.
Together, these findings establish key prerequisites for employing calcium phosphate microrods as a biodegradable pulmonary carrier plat-form in subsequent studies incorporating therapeutic cargo.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE317nnn/GSE317613 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE317613 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1414546 ncbi.nlm.nih.gov/bioproject/PRJNA1414546 ↗
project · from NCBI GEO
- PubMed 42076080 pubmed.ncbi.nlm.nih.gov/42076080 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Biochemistry and cell biology 69% · RNA sequencing 65%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE317613 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].anzsrc:group:3101 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (69%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |