Omics · study · 2026
Itga8+ MSCs in the endosteal niche contribute to the maintenance of hematopoietic stem cell self-renewal capacity.
Listed in NCBI GEO
The bone marrow (BM) niche is a specialized microenvironment critical for the maintenance and regulation of hematopoietic stem cells (HSCs).
Description
Different niches in the BM support HSCs, and among them we analyzed the endosteal niche. The endosteal niche is formed by Alcam+ Sca-1- osteoblasts, Alcam- Sca-1+ mesenchymal stem cells (MSCs), and Alcam- Sca-1- cells.
Using single-cell gene expression analysis, we identified a unique subpopulation within the Alcam+ Sca-1- bone-lining cells (BLCs) that lacked osteoblast markers. This subpopulation was found to be a novel MSC with specific expression of the 8-integrin (Itga8). We compared gene expression between Itga8+ Alcam+ Sca-1- MSCs (Itga8+ MSCs), Itga8- Alcam+ Sca-1- BLCs, Alcam- Sca-1+ MSCs, and CD51+ CD140a+ MSCs to investigate the characteristics of Itga8+ MSCs.
Read the rest (1 more)
Itga8+ MSCs showed a distinct gene expression profile compared with the others, demonstrating that Itga8+ MSCs are different from previously reported BLCs and MSCs. Furthermore, Itga8+ MSCs were found to secrete extracellular matrix (ECM) that regulates HSCs, and therefore, Itga8+ MSCs are important MSCs that provide the ECM required to maintain HSCs within the endosteal niche.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE280nnn/GSE280158 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE280158 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1176567 ncbi.nlm.nih.gov/bioproject/PRJNA1176567 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE280158 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |