Data · study · 2026
Optic Nerve Head Spatial Transcriptomic Change in Non-Human Primate Early Experimental Glaucoma.
Listed in NCBI GEO
Purpose: To determine optic nerve head (ONH) laminar collagen and retrolaminar myelin expression change in non-human-primate (NHP) experimental-glaucoma (EG) using immunohistochemistry (IHC) and spatial transcriptomics.
Description
Methods
Unilateral EG NHPs (n=3) were perfusion fixed, ONHs were trephined, embedded in paraffin and serial sectioned for IHC and spatial transcriptomics. EG versus control eye retinal nerve fiber layer thickness (RNFLT) and axon loss within each region of study were estimated. Results: CNPase levels decrease in the retrolaminar region of the ONH in early EG by IHC.
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Multiple myelin genes are decreased in early NHP EG in the retrolaminar region. Inflammatory pathways were upregulated in the retrolaminar region as well. Among the top genes that were altered in the laminar region were collagen related genes and Transforming Growth Factor Beta 1 (TGFβ1).
Conclusions: Spatial transcriptomics analysis revealed a consistent downregulation of multiple myelin-related genes, while IHC confirmed a corresponding decrease in CNPase protein expression. Importantly, spatial transcriptomics identified differential profiles among the prelaminar, laminar, and retrolaminar ONH. Together, these findings highlight early myelin disruption and provide insights into the spatial and molecular dynamics of disease onset in NHP.
This work advances our understanding of glaucoma pathogenesis and lays the groundwork for developing novel therapeutic strategies.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE316nnn/GSE316553 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE316553 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1403376 ncbi.nlm.nih.gov/bioproject/PRJNA1403376 ↗
project · from NCBI GEO
- PubMed 41810892 pubmed.ncbi.nlm.nih.gov/41810892 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Homo sapiens · Other
- From keywords
- Life Sciences
- Inferred from text
- Disease 75%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE316553 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:disease | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:other | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |