Omics · study · 2026
Transcriptomic profiling of vemurafenib-resistant versus parental BRAF V600E mouse melanoma cells (5555 and 5555R)
Listed in NCBI GEO
Resistance to BRAF inhibitors (BRAFi) limits long-term clinical benefit in BRAF-mutant melanoma.
Description
To characterise tumour-intrinsic transcriptional changes associated with acquired BRAFi resistance, we performed bulk RNA-seq of the BRAF V600E mouse melanoma cell line 5555 and its vemurafenib-resistant derivative 5555R, generated in our laboratory by prolonged exposure to vemurafenib. Compared with parental cells, resistant cells showed enrichment of inflammatory and interferon-response, cytoskeletal/actomyosin, mechano-sensing and epithelial-to-mesenchymal transition programmes.
These data accompany a study showing that BRAFi-resistant melanoma cells reactivate actomyosin contractility and adopt an altered secretory phenotype with increased M-CSF, which promotes differentiation of monocytes into immunosuppressive tumour-associated macrophages.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE348nnn/GSE348610 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE348610 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1534932 ncbi.nlm.nih.gov/bioproject/PRJNA1534932 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Bioinformatics and computational biology 68% · RNA sequencing 65%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE348610 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].anzsrc:group:3102 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (68%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |