Omics · study · 2026
Mechanical stress suppressed miR-330 in the development of osteoarthritis and its protection from osteochondral degeneration
Listed in NCBI GEO
Abnormal mechanical stress plays a crucial role in the progression of osteoarthritis (OA).
Description
However, more key factors regulating mechanical response remain to be explored. This study harvested temporomandibular joint synovium fluids from 96 patients and revealed that miR-330 is significantly reduced under abnormal mechanical stress.
Similarly, the expression of miR-330 is also decreased in cartilage and subchondral bone tissue from clinical specimen and animal models. Global knock out of miR-330 aggravated osteoarthritis development in both temporomandibular joint and knee joint. Conditional knock out of miR-330 in either chondrocytes or osteoclasts exhibited similar phenotype in both mechanical instable and aging animal models, suggesting the importance of miR-330 in both types of cells.
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Further molecular mechanism exploration unveiled that miR-330 can regulate the catabolism of chondrocytes and osteoclasts via suppressing CTGF, FGFR1 and EPOR as well as key inflammatory cytokines such as IL-1β and TNF-α. More importantly, intra-articular supplement of miR-330 exerts a therapeutic effect by mitigating the detrimental impacts of abnormal mechanical stress, where inhibition of chondrocytes apoptosis, osteoclasts activation and inflammation were further confirmed via single-cell RNA sequencing analysis.
In conclusion, this study for the first time revealed that miR-330 is a mechanical responsive, osteoarthritis protective micro RNA. We provide evidence that miR-330 has potential diagnostic and therapeutic value for both temporomandibular and knee osteoarthritis.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE307nnn/GSE307091 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE307091 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1314249 ncbi.nlm.nih.gov/bioproject/PRJNA1314249 ↗
project · from NCBI GEO
- PubMed 42212804 pubmed.ncbi.nlm.nih.gov/42212804 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Rattus norvegicus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE307091 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10116 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |