Omics · study · 2026
Transcriptomic profiling of a human iPSC-derived blood-brain barrier-on-chip model of ischemic stroke
Listed in NCBI GEO
Ischemic stroke is a leading cause of death and disability worldwide, characterized by reduced cerebral perfusion and blood-brain barrier (BBB) disruption.
Description
To model the human-specific pathophysiology of ischemic stroke, we developed a fully iPSC-derived blood-brain barrier-on-chip (iBBB-on-chip) comprising brain microvascular endothelial cells (iBMECs), pericytes (iPericytes), and astrocytes (iAstrocytes). After establishing a functional BBB structure, we induced ischemic injury by exposing the chip to oxygen-glucose deprivation (OGD) under static conditions.
Transcriptome profiling of brain side ande endothelial side was performed to investigate molecular responses to ischemic stress.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE294nnn/GSE294722 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE294722 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1251505 ncbi.nlm.nih.gov/bioproject/PRJNA1251505 ↗
project · from NCBI GEO
- PubMed 41221669 pubmed.ncbi.nlm.nih.gov/41221669 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE294722 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |