Omics · study · 2026
m6Am-seq profiling of macrophage-specific Pcif1 knockout mice during experimental colitis
Listed in NCBI GEO
Inflammatory bowel disease (IBD) is characterized by chronic intestinal inflammation accompanied by alterations in immune cell function and enteric nervous system homeostasis.
Description
To investigate the role of PCIF1-mediated N6,2′-O-dimethyladenosine (m6Am) RNA methylation in macrophages during colitis, we generated macrophage-specific Pcif1 knockout mice and performed transcriptome-wide RNA sequencing (RNA-seq) and m6Am profiling (m6Am-seq).
These datasets were generated to characterize m6Am-regulated gene expression programs and identify molecular pathways associated with extracellular matrix remodeling, macrophage function, and enteric neuronal regulation during intestinal inflammation.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE334nnn/GSE334689 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE334689 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1476171 ncbi.nlm.nih.gov/bioproject/PRJNA1476171 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus · Other
- From keywords
- Life Sciences
- Inferred from text
- Disease 75% · RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE334689 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:disease | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[method].geo_series_type:other | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |