Table 1_HIST1H2BJ is associated with poor prognosis and an immune-altered tumor microenvironment in glioma.xlsx

Additionally, potential small-molecule compounds were screened via molecular docking, and the binding stability was evaluated by molecular dynamics (MD) simulations.</p>Results<p>HIST1H2BJ was identified as a critical gene associated with glioma prognosis. Functional assays confirmed that HIST1H2BJ knockdown promoted cell apoptosis and inhibited cell invasion and migration. Glioma patients with high HIST1H2BJ expression were closely associated with an immunosuppressive tumor microenvironment, with significantly increased infiltration of M2 macrophages and regulatory T cells (Tregs), as well as upregulated immune checkpoint expression.

The IMPMG model, integrating HIST1H2BJ, immune-metabolic genes, and clinical variables, demonstrated superior prognostic accuracy (AUC > 0.86) and clinical utility compared to existing models. Furthermore, molecular docking identified VX-680 and A.770041 as candidate compounds associated with HIST1H2BJ-high drug-sensitivity prediction; their direct binding to HIST1H2BJ was assessed only in silico by molecular docking and dynamics simulations and requires experimental validation.</p>Conclusion<p>This study establishes HIST1H2BJ as an independent prognostic factor for glioma patient survival and a candidate prognostic biomarker whose therapeutic relevance requires further functional and preclinical investigation.</p>