Omics · study · 2026
CUT & RUN Analysis in Satellite Cells 7 days after Cardiotoxin Injury of Skeletal Muscle
Listed in NCBI GEO
The aim of this project is to assess the open chromatin regions, as well as the androgen (AR) and the glucocorticoid (GR) targets in satellite cells 7 days after cardiotoxin injury.
Description
Satellite cells were FACS-isolated and then lysed with Triton X-100 and released nuclei were bound to concanavalin-A magnetic beads. Nuclei/bead complexes were incubated with antibodies directed against AR, GR, a histone mark H3K4me2 and an IgG.
After wash, nuclei/bead complexes were incubated with A-micrococcal nuclease and chromatin cleavage was initiated with CaCl2.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE303nnn/GSE303618 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE303618 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1295933 ncbi.nlm.nih.gov/bioproject/PRJNA1295933 ↗
project · from NCBI GEO
Topics
- Stated by source
- Genome binding/occupancy profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE303618 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:genome-binding-occupancy-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |