ChIL-seq profiling of H3.3 and H3mm15 chromatin incorporation after histone chaperone depletion and amino acid substitution in NIH3T3 cells

To investigate the molecular mechanisms governing H3mm15 deposition and genomic localization, we performed Chromatin Integration Labeling followed by sequencing (ChIL-seq) in NIH3T3 cells expressing EGFP-tagged H3.3, H3mm15, or H3mm15 single-reversion mutants. The dataset consists of two complementary experiments.

First, H3.3-EGFP- and H3mm15-EGFP-expressing cells were treated with siRNAs targeting the histone chaperones Daxx or Hira and compared with cells treated with a non-targeting control siRNA. The genomic incorporation of the EGFP-tagged histones was profiled by GFP-ChIL-seq. Second, the genomic distributions of H3mm15-EGFP and three single-reversion mutants, Q100L, V112I, and C128R, were compared with that of H3.3-EGFP.