Supplementary file 1_Modulation of cell migration and invasion of thyroid cancer cells using miRNA-enriched extracellular vesicles.docx

However, their use as a delivery system in papillary thyroid carcinoma (PTC) remains underexplored. PTC is the most prevalent subtype of thyroid cancer, and despite its good prognosis, a fraction of the cases evolve into aggressive and fatal forms.

Here, we investigated whether non-tumor thyroid cells can be engineered to produce EVs-enriched with tumor suppressor miRNAs that could be delivered to PTC cells.</p>Methods<p>EVs from NThy-ORI cells transfected with miR-485-5p or miR-495-3p were isolated by differential ultracentrifugation and characterized by Nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), and Western Blot. Functional in vitro assays were conducted to explore the impact of miRNA-enriched EVs on PTC progression.</p>Results<p>Non-tumor thyroid NThy-ORI cells overexpressing miR-485-5p or miR-495-3p efficiently produced EVs enriched with these miRNAs, which were internalized by PTC cells.