mRNA 3ʹ UTRs direct microRNA degradation to participate in imprinted gene networks and regulate growth dataset 1

One site in Atp6v1g1 and two in Lpar4 direct degradation of miR-335-3p, showing that in mammals, two sites in the same transcript, and multiple sites in different transcripts, can collaborate to destabilize a microRNA. Moreover, sites in Plagl1 and Lrrc58 direct degradation of miR-322 and miR-503, respectively. Mice lacking the Plagl1 and Lrrc58 sites were smaller, demonstrating that target-directed degradation of miR-322 and miR-503 promotes growth.

Both miR-335-3p and Plagl1 are maternally imprinted, implying their participation in parental conflict, but their corresponding triggers or target microRNA partner are not imprinted. Thus, 3¢ UTRs can participate in parental conflict not only by regulating protein production but also directly by engaging TDMD to access an additional layer of regulation within a network of imprinted and biallelic genes.